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MANUEL
POMERANZ, PhD
CURRICULUM VITAE ET STUDIORUM
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EDUCATION
AND RESEARCH EXPERIENCE:
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| March 2012-July 2013 |
Associate Scientist – Cell Research Laboratory
Department of Biological System Research
Philip Morris International R&D |
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| Sept. 2009- Jan. 2012 |
Scientific Consultant for Unimed Scientifica S.r.l.
Product Specialist/Manager for foreign companies/products represented (Geneaid, Origene, Vilber Lourmat, Accurate). Commercial Manager for South Rome customers |
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April 2006 -
September 2009 |
Senior Research Biologist in IRBM-Merck MRL in Pomezia.
Supervisor of the Automated Cell Culture Laboratory, “Biotechnology” department.
Supervisor: Doct. Monaci |
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| January 2008 |
Training on Pichia Pastoris System (Glycofi, Lebanon NH) |
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October 2004-
April 2006 |
Research Biologist in IRBM-Merck MRL in Pomezia. Worked in the “Antiviral” department.
Supervisor: Doct. Nicosia |
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| October 2001-2004 |
PhD student (Cellular and Molecular Biology Program, University of Tor Vergata, Rome) in the Pharmacology Department of IRBM-MERCK
(Rome).
Thesis title: "In-vitro and in-vivo characterization
of human liver transporters MRP2 and LST1 using Adenovirus vectors",
supervisors Doct. Laufer and Doct. Demartis. |
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| September 2000 |
1 year fellowship in IRBM-MERCK in the Pharmacology
Department.
Project: “Study and characterization of human liver transporters”. |
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| 1999/2000 |
1 year of military service in CRSSA (Centre de Recherches et
du Service de Santé des Armées, Grenoble, France),
in the Immunology department. Project title: "Set-up of
a rapid diagnostic kit for the detection of yellow fever".
Supervisor: Doct. Thullier Director: Doct. Attree |
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| September 1999 |
DEA (Diplôme d'Etudes Approfondies) in Pharmacology, Molecular and Cellular Biology,
at IPMC (Institut de Pharmacologie Moléculaire et Cellulaire, UPR 441, Director Prof. Michel Lazdunski, Sophia-Antipolis, Nice, France), discussing an experimental thesis: “Study of the signal pathway and physiologic role of soluble Fractalkine in MonoMac6 cells”. |
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| April 1999 |
Stage in the Microbiology department at "Istituto di Microbiologia dell'Università Cattolica del Sacro Cuore" (Rome)
Supervisor: Doct. Cattani Director: Doct. Fadda |
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September 1998-
September
1999 |
1 year fellowship in the Immunology department of INSERM (Institut National de la Santé et de la Recherche Médicale, Unit364) at the Faculty of Medicine in Nice, France.
Supervisor: Doct. Schmid Director: Doct. Rossi |
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| June 1998 |
University Degree in Biological Science (Biochemist course), University of Nice Sophia-Antipolis (France) |
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TECHNICAL EXPERTISE
| AUTOMATION |
- SelecT, Cello workstations (Cell culture automation, The Automation Partnership)
- ORCA, FX, NX workstations (High Throughput production of proteins, Beckman)
- Multiprobe II & AKTA (HT purification of proteins, Perkin Elmer &GE)
- Bioanalyzer & Octet Systems (Proteins QC, Agilent &ForteBio) |
| IN VITRO |
- Eukaryotic/prokaryotic cell cultures (Primary, immortalized, bacteria and yeast)
- Antibody production, purification and characterization
- Transient and stable transfection of eukaryotic cells
- Adenovirus construction, growth and purification
- Infection of eukaryotic cells with Adenovirus
.... (purification, genomic structure analysis, quantitative PCR (TaqMan), scale-up production)
- Analysis of protein expression in transfected/infected cells
.... (western blotting, immunocytochemistry, immunofluorescence, dot blots)
- Molecular biology of nucleic acids (DNA, RNA)
- ELISA Assay
- MAP kinase activity assay
- Cell migration assay
- Uptake assay in eukaryotic cells of radio labeled substrates
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| IN
VIVO |
- Endovenous and intraperitoneal infection of rodent animals with Adenovirus
- Good experience in Animal Care ( administrations, incanulations, necroscopies...) with successive
......analysis of protein, DNA and RNA expression in tissues ( Western Blot, Immunohistochemistry,
......TaqMan, Immunofluorescence ) |
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| Spoken Languages: |
- Italian and French (mother tongue),
- English (Proficient User C1 (COE European Framework).)
- Spanish (basal) |
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| Computer skills: |
- Windows (95, 98, Millennium, XP, Vista, Windows 7), Macintosh, MS Office (Word,
Excel, PowerPoint, And Outlook), Languages (Dos, HTML), Explorer, Chrome, Various programs (Dreamweaver, Photoshop, Flash, Kaleidagraph,
Vector NTI, SDS 2.0…) |
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Hobbies: |
Soccer, Tennis, Travel , Internet (webmaster experiences), historical
cars (attend meetings) |
| LIST OF PUBLICATIONS
1) Cambien B, Pomeranz M, Millet MA, Rossi B, Schmid-Alliana
A.
Signal transduction involved in MCP-1-mediated monocytic
transendothelial migration.
Blood. 2001 Jan 15;97(2):359-66.
2) Cambien B, Pomeranz M, Schmid-Antomarchi H, Millet
MA, Breittmayer V, Rossi B, Schmid-Alliana A.
Signal transduction pathways involved in soluble fractalkine-induced
monocytic cell adhesion.
Blood. 2001 Apr 1;97(7):2031-7.
3) Vitale S, Schmid-Alliana A, Breuil V, Pomeranz
M, Millet MA, Rossi B, Schmid-Antomarchi H.
Soluble fractalkine prevents monocyte chemoattractant protein-1-induced
monocyte migration via inhibition of stress-activated protein
kinase 2/p38 and matrix metalloproteinase activities.
J Immunol. 2004 Jan 1;172(1):585-92.
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LINKS
IRBM
UNICE
CRSSA
Università
degli Studi di Tor Vergata
IPMC
Università
cattolica
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DATA PROCESSING CONSENT AGREEMENT
Fully informed of my rights as stated in art. 7 of Dlgs
30/06/03 n. 196, and aware of my freedom of choice,
I hereby expressly agree and consent to the processing
of all of my personal data contained in the Curriculum
Vitae enclosed, that I have spontaneously sent to the
addressee.
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